CoraLite® Plus 488 Anti-Human CD86 Rabbit Recombinant Antibody
CD86 Uni-rAbTM Recombinant Antibody for FC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240429C6
验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
For other applications, we recommend the unconjugated version of this antibody, 98043-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL488-98043 is CD86 Fusion Protein expressed in E. coli.
| Tested Applications | FC Application Description |
| Tested Reactivity | human |
| Immunogen | Fusion Protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | CD86 molecule |
| Synonyms | BU63, B70, B7 2, Activation B7-2 antigen, Activation B7 2 antigen |
| Calculated Molecular Weight | 329 aa, 38 kDa |
| GenBank Accession Number | NM_175862 |
| Gene Symbol | CD86 |
| Gene ID (NCBI) | 942 |
| ENSEMBL Gene ID | ENSG00000114013 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P42081 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD86 antibody CL488-98043 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |