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PE Anti-Human CD86 Rabbit Recombinant Antibody

CD86 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

240429C6

Cat no : PE-98043

Print datasheet

Synonyms

BU63, B70, B7 2, Activation B7-2 antigen, Activation B7 2 antigen

验证数据展示

  • FC experiment of human PBMCs using PE-98043

    FC experiment of human PBMCs using PE-98043

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody, and 5 ul PE Anti-Human CD86 Rabbit Recombinant Antibody (PE-98043, Clone: 240429C6) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using PE-98043

    FC experiment of human PBMCs using PE-98043

    1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD86 Rabbit Recombinant Antibody (PE-98043, Clone: 240429C6) (red) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-98043 targets CD86 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD86 molecule
Synonyms BU63, B70, B7 2, Activation B7-2 antigen, Activation B7 2 antigen
Calculated Molecular Weight 329 aa, 38 kDa
GenBank Accession NumberNM_175862
Gene Symbol CD86
Gene ID (NCBI) 942
ENSEMBL Gene IDENSG00000114013
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42081
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

实验方案

Product Specific Protocols
FC protocol for PE CD86 antibody PE-98043Download protocol
Standard Protocols
Click here to view our Standard Protocols