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CoraLite®594 Anti-Human CD86 (BU63)

CD86 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

BU63

Cat no : CL594-65165

Print datasheet

Synonyms

CD28LG2, BU63, B70, B7 2, Activation B7-2 antigen

验证数据展示

  • FC experiment of human PBMCs using CL594-65165

    FC experiment of human PBMCs using CL594-65165

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD86 (CL594-65165, Clone:BU63) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21) and CL750 Anti-Human CD14 (CL750-65246). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using CL594-65165

    FC experiment of human PBMCs using CL594-65165

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD86 (CL594-65165, Clone:BU63) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65165-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65165 targets CD86 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen B-lymphoblastoid cell line ARH 77 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD86 molecule
Synonyms CD28LG2, BU63, B70, B7 2, Activation B7-2 antigen
Calculated Molecular Weight 329 aa, 38 kDa
GenBank Accession NumberBC040261
Gene Symbol CD86
Gene ID (NCBI) 942
ENSEMBL Gene IDENSG00000114013
RRIDAB_2883470
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP42081
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

实验方案

Product Specific Protocols
FC protocol for CL594 CD86 antibody CL594-65165Download protocol
Standard Protocols
Click here to view our Standard Protocols