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100 ul human peripheral blood were surface stained with 10 ul FITC-Anti-Human CD14 (FITC-65056, Clone: UCHM-1), and 5 ul CoraLite®594-conjugated Anti-Human CD86 (CL594-65165, Clone: BU63) or CoraLite®594-conjugated Mouse IgG1 isotype control. Cells were then treated with red blood cell lysis buffer and were gated for CD14+ monocytes for analysis of CD86 staining.
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.