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CoraLite® Plus 405 Anti-Human CD8a (RPA-T8)

CD8a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

RPA-T8

Cat No : CL405-65144

Print datasheet

Synonyms

CD8, RPA-T8, p32, MAL, Leu2

验证数据展示

  • FC experiment of human PBMCs using CL405-65144

    FC experiment of human PBMCs using CL405-65144

    1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 405 Anti-Human CD8a (RPA-T8) (CL405-65144, Clone: RPA-T8) or CoraLite® Plus 405 Mouse IgG1 Isotype Control (MOPC-21) (CL405-65124, Clone: MOPC-21). Cells were not fixed.

  • FC experiment of human PBMCs using CL405-65144

    FC experiment of human PBMCs using CL405-65144

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD8a (CL405-65144, Clone:RPA-T8) (red) or CoraLite® Plus 405 Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65144-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL405-65144 targets CD8a in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD8a molecule
Synonyms CD8, RPA-T8, p32, MAL, Leu2
Calculated Molecular Weight 235 aa, 26 kDa
GenBank Accession NumberBC025715
Gene Symbol CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
Conjugate CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission Maxima Wavelengths399 nm / 422 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01732
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

背景介绍

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

实验方案

Product Specific Protocols
FC protocol for CL Plus 405 CD8a antibody CL405-65144Download protocol
Standard Protocols
Click here to view our Standard Protocols