CoraLite® Plus 594 Anti-Human CD8a (RPA-T8)
CD8a Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
human
Applications
FC
Conjugate
CoraLite® Plus 594 Fluorescent Dye
CloneNo.
RPA-T8
验证数据展示
经过测试的应用
Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65144-1-Ig
推荐稀释比
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-65144 targets CD8a in FC applications and shows reactivity with human samples.
Tested Applications | FC Application Description |
Tested Reactivity | human |
Immunogen | N/A 种属同源性预测 |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Full Name | CD8a molecule |
Synonyms | CD8, RPA-T8, p32, MAL, Leu2 |
Calculated Molecular Weight | 235 aa, 26 kDa |
GenBank Accession Number | BC025715 |
Gene Symbol | CD8A |
Gene ID (NCBI) | 925 |
ENSEMBL Gene ID | ENSG00000153563 |
Conjugate | CoraLite® Plus 594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 594 nm / 615 nm |
Form | Liquid |
Purification Method | Affinity purification |
UNIPROT ID | P01732 |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. |
背景介绍
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 594 CD8a antibody CL594-65144 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |