CoraLite® Plus 488 Anti-Rat CD8a (OX-8)
CD8a Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Rat
Applications
FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
OX-8
验证数据展示
经过测试的应用
| Positive FC detected in | rat splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65180-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65180 targets CD8a in FC applications and shows reactivity with Rat samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | Rat |
| Immunogen | High-molecular-weight rat thymocyte glycoproteins 种属同源性预测 |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD8a molecule |
| Synonyms | Cd8a, CD8a molecule |
| GenBank Accession Number | BC088126 |
| Gene Symbol | Cd8a |
| Gene ID (NCBI) | 24930 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Purified by protein-A affinity chromatography |
| UNIPROT ID | P07725 |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD8a antibody CL488-65180 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
