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PE Anti-Rat CD8a (OX-8)

CD8a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Rat

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

OX-8

Cat no : PE-65180

Print datasheet

Synonyms

Cd8a, CD8a molecule

验证数据展示

  • FC experiment of rat splenocytes using PE-65180

    FC experiment of rat splenocytes using PE-65180

    1X10^6 rat splenocytes were surface co-stained with APC Anti-Rat CD4 (OX-35) and 0.2 ug PE Anti-Rat CD8a (PE-65180, Clone: OX-8) or 0.2 ug PE Mouse IgG1 Isotype Control (MOPC-21) (PE-65124, Clone: MOPC-21). Cells were not fixed.

经过测试的应用

Positive FC detected inrat splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65180 targets CD8a in FC applications and shows reactivity with Rat samples.

Tested Applications FC Application Description
Tested Reactivity Rat
Immunogen High-molecular-weight rat thymocyte glycoproteins 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD8a molecule
Synonyms Cd8a, CD8a molecule
GenBank Accession NumberBC088126
Gene Symbol Cd8a
Gene ID (NCBI) 24930
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDP07725
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

实验方案

Product Specific Protocols
FC protocol for PE CD8a antibody PE-65180Download protocol
Standard Protocols
Click here to view our Standard Protocols