验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
APC-98095 targets CD9 in FC applications and shows reactivity with human samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | human |
| Immunogen | Recombinant protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | CD9 molecule |
| Synonyms | CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9 |
| Calculated Molecular Weight | 25 kDa |
| GenBank Accession Number | NM_001769.4 |
| Gene Symbol | CD9 |
| Gene ID (NCBI) | 928 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P21926 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, it may be due to the difference of glycosylations (PMID: 8701996).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD9 antibody APC-98095 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |