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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using CL594-98095

    FC experiment of human PBMCs using CL594-98095

    1x10^6 human PBMCs were surface stained with APC Anti-Human CD3 and 5 ul CoraLite®594 Anti-Human CD9 Rabbit Recombinant Antibody (CL594-98095, Clone:240830E6), or CoraLite®594 Rabbit IgG Isotype Control Recombinant Antibody (CL594-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using CL594-98095

    FC experiment of human PBMCs using CL594-98095

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD9 Rabbit Recombinant Antibody (CL594-98095, Clone:240830E6)(red), or CoraLite®594 Rabbit IgG Isotype Control Recombinant Antibody (CL594-98136, Clone: 240953C9)(blue). Cells were incubated with FC Receptor Block prior to staining.Cells were not fixed.

CoraLite®594 Anti-Human CD9 Rabbit Recombinant Antibody

CD9 Uni-rAbTM Recombinant Antibody for FC
Cat No. CL594-98095

产品说明书

CloneNo. 240830E6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 98095-1-PBS

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-98095 targets CD9 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD9 molecule
别名 CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9
计算分子量25 kDa
GenBank蛋白编号NM_001769.4
基因名称 CD9
Gene ID (NCBI) 928
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21926
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, it may be due to the difference of glycosylations (PMID: 8701996).

实验方案

Product Specific Protocols
FC protocol for CL594 CD9 antibody CL594-98095Download protocol
Standard Protocols
Click here to view our Standard Protocols