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验证数据展示

  • FC experiment of human PBMCs using CL594-98095

    FC experiment of human PBMCs using CL594-98095

    1x10^6 human PBMCs were surface stained with APC Anti-Human CD3 and 5 ul CoraLite®594 Anti-Human CD9 Rabbit Recombinant Antibody (CL594-98095, Clone:240830E6), or CoraLite®594 Rabbit IgG Isotype Control Recombinant Antibody (CL594-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using CL594-98095

    FC experiment of human PBMCs using CL594-98095

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD9 Rabbit Recombinant Antibody (CL594-98095, Clone:240830E6)(red), or CoraLite®594 Rabbit IgG Isotype Control Recombinant Antibody (CL594-98136, Clone: 240953C9)(blue). Cells were incubated with FC Receptor Block prior to staining.Cells were not fixed.

CoraLite®594 Anti-Human CD9 Rabbit Recombinant Antibody

CD9 Uni-rAbTM Recombinant Antibody for FC
Cat No. CL594-98095

Print datasheet

CloneNo. 240830E6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 98095-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-98095 targets CD9 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD9 molecule
Synonyms CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9
Calculated Molecular Weight25 kDa
GenBank Accession NumberNM_001769.4
Gene Symbol CD9
Gene ID (NCBI) 928
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21926
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, it may be due to the difference of glycosylations (PMID: 8701996).

实验方案

Product Specific Protocols
FC protocol for CL594 CD9 antibody CL594-98095Download protocol
Standard Protocols
Click here to view our Standard Protocols