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  • Featured Product
  • KD/KO Validated

Biotin-conjugated CD9 Monoclonal antibody

CD9 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC

Conjugate

Biotin

CloneNo.

4H7B9

Cat no : Biotin-60232

Print datasheet

Synonyms

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, DRAP 27, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29

验证数据展示

  • IHC staining of human ovary tumor using Biotin-60232

    IHC staining of human ovary tumor using Biotin-60232

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using Biotin-60232 (CD9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human ovary tumor using Biotin-60232

    IHC staining of human ovary tumor using Biotin-60232

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using Biotin-60232 (CD9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD9 antibody included

经过测试的应用

Positive IHC detected inhuman ovary tumor tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-60232 targets CD9 in IHC applications and shows reactivity with human samples.

Tested Applications IHC Application Description
Tested Reactivity human
Immunogen CD9 fusion protein Ag14529 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD9 molecule
Synonyms 5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, DRAP 27, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29
Calculated Molecular Weight 228 aa, 25 kDa
Observed Molecular Weight 23-27 kDa
GenBank Accession NumberBC011988
Gene Symbol CD9
Gene ID (NCBI) 928
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21926
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.

实验方案

Product Specific Protocols
IHC protocol for Biotin CD9 antibody Biotin-60232Download protocol
Standard Protocols
Click here to view our Standard Protocols