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验证数据展示

  • IHC staining of human ovary tumor using Biotin-60232

    IHC staining of human ovary tumor using Biotin-60232

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using Biotin-60232 (CD9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human ovary tumor using Biotin-60232

    IHC staining of human ovary tumor using Biotin-60232

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using Biotin-60232 (CD9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD9 antibody included
  • Featured Product
  • KD/KO验证

Biotin-conjugated CD9 Monoclonal antibody

CD9 Monoclonal Antibody for IHC
Cat No. Biotin-60232

产品说明书

CloneNo. 4H7B9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC

别名

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, DRAP 27, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman ovary tumor tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-60232 targets CD9 in IHC applications and shows reactivity with human samples.

经测试应用 IHC Application Description
经测试反应性 human
免疫原 CD9 fusion protein Ag14529 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD9 molecule
别名 5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, DRAP 27, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29
计算分子量 228 aa, 25 kDa
观测分子量 23-27 kDa
GenBank蛋白编号BC011988
基因名称 CD9
Gene ID (NCBI) 928
RRIDAB_3083811
偶联类型 Biotin
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP21926
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.

实验方案

Product Specific Protocols
IHC protocol for Biotin CD9 antibody Biotin-60232Download protocol
Standard Protocols
Click here to view our Standard Protocols