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- KD/KO Validated
CoraLite® Plus 488-conjugated CD9 Monoclonal antibody
CD9 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human, rat
Applications
IF-P and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4H7B9
验证数据展示
经过测试的应用
| Positive IF-P detected in | human lung cancer tissue, human breast cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60232-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-60232 targets CD9 in IF-P applications and shows reactivity with human, rat samples.
| Tested Applications | IF-P Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Immunogen | CD9 fusion protein Ag14529 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD9 molecule |
| Synonyms | 5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, Cell and organelle markers, DRAP 27, Exosome marker, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29 |
| Calculated Molecular Weight | 228 aa, 25 kDa |
| Observed Molecular Weight | 23-27 kDa |
| GenBank Accession Number | BC011988 |
| Gene Symbol | CD9 |
| Gene ID (NCBI) | 928 |
| RRID | AB_2919239 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P21926 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD9 antibody CL488-60232 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
