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APC Anti-Mouse CD90.2 (30-H12)

CD90.2 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

30-H12

Cat no : APC-65088

Print datasheet

Synonyms

CD90, T25, Thy 1, Thy 1.2, Thy1, Thy1.1, Thy1.2, thymus cell antigen 1, theta

验证数据展示

  • FC experiment of C57BL/6 mouse thymocytes using APC-65088

    FC experiment of C57BL/6 mouse thymocytes using APC-65088

    1X10^6 C57BL/6 mouse thymocytes were surface stained with 0.2 ug APC Anti-Mouse CD90.2 (APC-65088, Clone: 30-H12) or 0.2 ug Isotype Control. Cells were not fixed.

经过测试的应用

Positive FC detected inC57BL/6 mouse thymocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65088 targets CD90.2 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen Mouse thymus or spleen 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name thymus cell antigen 1, theta
Synonyms CD90, T25, Thy 1, Thy 1.2, Thy1, Thy1.1, Thy1.2, thymus cell antigen 1, theta
GenBank Accession NumberBC054436
Gene Symbol CD90
Gene ID (NCBI) 21838
RRIDAB_2882983
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01831
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR (PMID: 5919593).

实验方案

Product Specific Protocols
FC protocol for APC CD90.2 antibody APC-65088Download protocol
Standard Protocols
Click here to view our Standard Protocols