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CoraLite® Plus 488 Anti-Mouse CD90.2 (30-H12)

CD90.2 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

30-H12

Cat no : CL488-65088

Print datasheet

Synonyms

CD90, CD90.2, T25, Thy 1, Thy 1.2, Thy1, Thy1.2, thymus cell antigen 1, theta

验证数据展示

  • FC experiment of mouse splenocytes using CL488-65088

    FC experiment of mouse splenocytes using CL488-65088

    1X10^6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD3ε (145-2C11) and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD90.2 (CL488-65088, Clone:30-H12) or 0.25 ug isotype control. Cells were not fixed.

  • IF Staining of mouse splenocytes using CL488-65088

    IF Staining of mouse splenocytes using CL488-65088

    Immunofluorescent analysis of BALB/c mouse splenocytes using CoraLite® Plus 488 CD90.2 antibody (CL488-65088, Clone: 30-H12 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65088-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65088 targets CD90.2 in IF/ICC, FC applications and shows reactivity with Mouse samples.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity Mouse
Immunogen Mouse thymus or spleen 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name thymus cell antigen 1, theta
Synonyms CD90, CD90.2, T25, Thy 1, Thy 1.2, Thy1, Thy1.2, thymus cell antigen 1, theta
GenBank Accession NumberBC054436
Gene Symbol CD90
Gene ID (NCBI) 21838
RRIDAB_2883170
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR (PMID: 5919593).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD90.2 antibody CL488-65088Download protocol
FC protocol for CL Plus 488 CD90.2 antibody CL488-65088Download protocol
Standard Protocols
Click here to view our Standard Protocols