• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Mouse CD90.2 (30-H12)

CD90.2 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

Unconjugated

CloneNo.

30-H12

Cat No : 65088-1-Ig

Print datasheet

Synonyms

CD90, CD90.2, Thy 1, Thy 1.2, Thy1

验证数据展示

  • FC experiment of mouse thymocytes using 65088-1-Ig

    FC experiment of mouse thymocytes using 65088-1-Ig

    1X10^6 BALB/c mouse thymocytes were surface stained with 1 ug Anti-Mouse CD90.2 (65088-1-Ig, Clone: 30-H12) and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rat IgG(H+L) at dilution 1:1000 (green), or stained with 1 ug Rat IgG2b Isotype Control and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rat IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse thymocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

65088-1-Ig targets CD90.2 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Cited ApplicationsFC
Tested Reactivity Mouse
Cited Reactivityrat
Immunogen N/A 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name thymus cell antigen 1, theta
Synonyms CD90, CD90.2, Thy 1, Thy 1.2, Thy1
GenBank Accession NumberBC054436
Gene Symbol Thy1
Gene ID (NCBI) 21838
RRIDAB_2918391
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR (PMID: 5919593).

实验方案

Product Specific Protocols
FC protocol for CD90.2 antibody 65088-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratFC

Regen Ther

METTL3 promotes SMSCs chondrogenic differentiation by targeting the MMP3, MMP13, and GATA3

Authors - Bin Hu
View Article