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CoraLite® Plus 488-conjugated CD98/SLC3A2 Monoclonal antibody

CD98/SLC3A2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B10F5

Cat no : CL488-66883

Print datasheet

Synonyms

CD98, SLC3A2, MDU1, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain

验证数据展示

  • IF Staining of HeLa using CL488-66883

    IF Staining of HeLa using CL488-66883

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 CD98/SLC3A2 antibody (CL488-66883, Clone: 2B10F5 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CD98/SLC3A2 Ready-To-Use IHC Kit. CD98/SLC3A2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66883-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66883 targets CD98/SLC3A2 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen CD98/SLC3A2 fusion protein Ag7301 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Synonyms CD98, SLC3A2, MDU1, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 85-94 kDa, 120-130 kDa
GenBank Accession NumberBC001061
Gene Symbol CD98
Gene ID (NCBI) 6520
RRIDAB_2919385
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08195
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD98/SLC3A2 antibody CL488-66883Download protocol
Standard Protocols
Click here to view our Standard Protocols