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验证数据展示

  • IF Staining of HeLa using CL594-66883

    IF Staining of HeLa using CL594-66883

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 CD98/SLC3A2 antibody (CL594-66883, Clone: 2B10F5 ) at dilution of 1:200.

CoraLite®594-conjugated CD98/SLC3A2 Monoclonal antibody

CD98/SLC3A2 Monoclonal Antibody for IF/ICC
Cat No. CL594-66883

产品说明书

CloneNo. 2B10F5

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC

别名

CD98, SLC3A2, MDU1, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CD98/SLC3A2 Ready-To-Use IHC Kit. CD98/SLC3A2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66883-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66883 targets CD98/SLC3A2 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 CD98/SLC3A2 fusion protein Ag7301 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
别名 CD98, SLC3A2, MDU1, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain
计算分子量 68 kDa
观测分子量 85-94 kDa, 120-130 kDa
GenBank蛋白编号BC001061
基因名称 CD98
Gene ID (NCBI) 6520
RRIDAB_2920052
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08195
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

实验方案

Product Specific Protocols
IF protocol for CL594 CD98/SLC3A2 antibody CL594-66883Download protocol
Standard Protocols
Click here to view our Standard Protocols