CDC25B Monoclonal antibody
CDC25B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1H3A4
验证数据展示
经过测试的应用
| Positive WB detected in | Sp2/0 cells, Jurkat cells, U-937 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy CDC25B Ready-To-Use IHC Kit. CDC25B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
67145-1-Ig targets CDC25B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | CDC25B fusion protein Ag27193 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cell division cycle 25 homolog B (S. pombe) |
| Synonyms | M phase inducer phosphatase 2, EC:3.1.3.48, Cell division cycle 25 homolog B, CDC25HU2, Cdc 25B |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 69 kDa |
| GenBank Accession Number | BC006395 |
| Gene Symbol | CDC25B |
| Gene ID (NCBI) | 994 |
| RRID | AB_2882444 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P30305 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC25B antibody 67145-1-Ig | Download protocol |
| IHC protocol for CDC25B antibody 67145-1-Ig | Download protocol |
| IF protocol for CDC25B antibody 67145-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
