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CoraLite®594-conjugated CDC25B Monoclonal antibody

CDC25B Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H3A4

Cat no : CL594-67145

Print datasheet

Synonyms

M phase inducer phosphatase 2, EC:3.1.3.48, Cell division cycle 25 homolog B, CDC25HU2, Cdc 25B

验证数据展示

  • IF Staining of Jurkat using CL594-67145

    IF Staining of Jurkat using CL594-67145

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite®594 CDC25B antibody (CL594-67145, Clone: 1H3A4 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL594-67145

    FC experiment of Jurkat using CL594-67145

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CDC25B (CL594-67145, Clone:1H3A4) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CDC25B Ready-To-Use IHC Kit. CDC25B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67145-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67145 targets CDC25B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CDC25B fusion protein Ag27193 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cell division cycle 25 homolog B (S. pombe)
Synonyms M phase inducer phosphatase 2, EC:3.1.3.48, Cell division cycle 25 homolog B, CDC25HU2, Cdc 25B
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC006395
Gene Symbol CDC25B
Gene ID (NCBI) 994
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30305
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

实验方案

Product Specific Protocols
IF protocol for CL594 CDC25B antibody CL594-67145Download protocol
FC protocol for CL594 CDC25B antibody CL594-67145Download protocol
Standard Protocols
Click here to view our Standard Protocols