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验证数据展示

  • WB analysis of Sp2/0 using 67145-1-Ig (same clone as 67145-1-PBS)

    WB analysis of Sp2/0 using 67145-1-Ig (same clone as 67145-1-PBS)

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 67145-1-Ig (CDC25B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 67145-1-Ig (same clone as 67145-1-PBS)

    IHC staining of human skin cancer using 67145-1-Ig (same clone as 67145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67145-1-Ig (CDC25B antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC25B antibody included
  • IF Staining of Jurkat using 67145-1-Ig (same clone as 67145-1-PBS)

    IF Staining of Jurkat using 67145-1-Ig (same clone as 67145-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CDC25B antibody (67145-1-Ig, Clone: 1H3A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

CDC25B Monoclonal antibody, PBS Only

CDC25B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67145-1-PBS

产品说明书

CloneNo. 1H3A4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

M phase inducer phosphatase 2, EC:3.1.3.48, Cell division cycle 25 homolog B, CDC25HU2, Cdc 25B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CDC25B Ready-To-Use IHC Kit. CDC25B primary antibody included.

产品信息

67145-1-PBS targets CDC25B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 CDC25B fusion protein Ag27193 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cell division cycle 25 homolog B (S. pombe)
别名 M phase inducer phosphatase 2, EC:3.1.3.48, Cell division cycle 25 homolog B, CDC25HU2, Cdc 25B
计算分子量 65 kDa
观测分子量 69 kDa
GenBank蛋白编号BC006395
基因名称 CDC25B
Gene ID (NCBI) 994
RRIDAB_2882444
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP30305
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.