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CDC25B Monoclonal antibody, PBS Only

CDC25B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H3A4

Cat no : 67145-1-PBS

Print datasheet

Synonyms

M phase inducer phosphatase 2, EC:3.1.3.48, Cell division cycle 25 homolog B, CDC25HU2, Cdc 25B

验证数据展示

  • WB analysis of Sp2/0 using 67145-1-Ig (same clone as 67145-1-PBS)

    WB analysis of Sp2/0 using 67145-1-Ig (same clone as 67145-1-PBS)

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 67145-1-Ig (CDC25B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 67145-1-Ig (same clone as 67145-1-PBS)

    IHC staining of human skin cancer using 67145-1-Ig (same clone as 67145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67145-1-Ig (CDC25B antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC25B antibody included
  • IF Staining of Jurkat using 67145-1-Ig (same clone as 67145-1-PBS)

    IF Staining of Jurkat using 67145-1-Ig (same clone as 67145-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CDC25B antibody (67145-1-Ig, Clone: 1H3A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CDC25B Ready-To-Use IHC Kit. CDC25B primary antibody included.

产品信息

67145-1-PBS targets CDC25B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen CDC25B fusion protein Ag27193 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cell division cycle 25 homolog B (S. pombe)
Synonyms M phase inducer phosphatase 2, EC:3.1.3.48, Cell division cycle 25 homolog B, CDC25HU2, Cdc 25B
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC006395
Gene Symbol CDC25B
Gene ID (NCBI) 994
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30305
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.