验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
| Positive FC (Intra) detected in | HeLa cells, HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84668-4-RR targets CEBP Alpha/CEBPA in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, FC (Intra), ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | CEBP Alpha/CEBPA fusion protein Ag29947 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | CCAAT/enhancer binding protein (C/EBP), alpha |
| Synonyms | CEBP alpha, CEBPA, CEBP, CCAAT/enhancer-binding protein alpha, C/EBPα |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | NM_004364 |
| Gene Symbol | CEBPA |
| Gene ID (NCBI) | 1050 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P49715 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CEBP Alpha/CEBPA antibody 84668-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
