验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, Jurkat cells |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11517-1-AP targets CENPJ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, zebrafish |
| 免疫原 | CENPJ fusion protein Ag2078 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | centromere protein J |
| 别名 | CPAP, LAG-3-associated protein, Centrosomal P4.1-associated protein, Centromere protein J, CENP-J |
| 计算分子量 | 1338 aa, 153 kDa |
| 观测分子量 | 153 kDa |
| GenBank蛋白编号 | BC024209 |
| 基因名称 | CENPJ |
| Gene ID (NCBI) | 55835 |
| RRID | AB_2244605 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HC77 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CENPJ antibody 11517-1-AP | Download protocol |
| IF protocol for CENPJ antibody 11517-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Cell biology. Reversible centriole depletion with an inhibitor of Polo-like kinase 4. | ||
Nat Cell Biol The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis. | ||
Neuron Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate. | ||
Dev Cell SAS-6 Assembly Templated by the Lumen of Cartwheel-less Centrioles Precedes Centriole Duplication. | ||
Nat Commun Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. |
