验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MDCK cells |
For other applications, we recommend the unconjugated version of this antibody, 11517-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-11517 targets CENPJ in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | CENPJ fusion protein Ag2078 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | centromere protein J |
| 别名 | BM032, CENP J, CENPJ, centromere protein J, CPAP, LAP, LIP1, LYST interacting protein 1, MCPH6 |
| 计算分子量 | 1338 aa, 153 kDa |
| GenBank蛋白编号 | BC024209 |
| 基因名称 | CENPJ |
| Gene ID (NCBI) | 55835 |
| RRID | AB_2919766 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HC77 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 CENPJ antibody CL594-11517 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |