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  • Featured Product
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CoraLite® Plus 488-conjugated CENPJ Polyclonal antibody

CENPJ Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11517

Print datasheet

Synonyms

BM032, CENP J, CENPJ, centromere protein J, CPAP, LAG 3 associated protein, LAP, LIP1, LYST interacting protein 1, MCPH6

验证数据展示

  • IF Staining of HeLa using CL488-11517

    IF Staining of HeLa using CL488-11517

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 CENPJ antibody (CL488-11517) at dilution of 1:600.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 11517-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11517 targets CENPJ in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen CENPJ fusion protein Ag2078 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centromere protein J
Synonyms BM032, CENP J, CENPJ, centromere protein J, CPAP, LAG 3 associated protein, LAP, LIP1, LYST interacting protein 1, MCPH6
Calculated Molecular Weight 1338 aa, 153 kDa
GenBank Accession NumberBC024209
Gene Symbol CENPJ
Gene ID (NCBI) 55835
RRIDAB_2919030
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HC77
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CENPJ antibody CL488-11517Download protocol
Standard Protocols
Click here to view our Standard Protocols