验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells, HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14498-1-AP targets CEP250/CNAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | CEP250/CNAP1 fusion protein Ag5925 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | centrosomal protein 250kDa |
| 别名 | Cep250, CEP250,C-NAP1, Centrosome-associated protein CEP250, centrosomal protein 250kDa, Centrosomal protein 2 |
| 计算分子量 | 281 kDa |
| 观测分子量 | 250 kDa |
| GenBank蛋白编号 | BC001433 |
| 基因名称 | CEP250/CNAP1 |
| Gene ID (NCBI) | 11190 |
| RRID | AB_2076918 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BV73 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP250, also known as C-Nap1, is a 250 kDa coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP250/CNAP1 antibody 14498-1-AP | Download protocol |
| IHC protocol for CEP250/CNAP1 antibody 14498-1-AP | Download protocol |
| IF protocol for CEP250/CNAP1 antibody 14498-1-AP | Download protocol |
| IP protocol for CEP250/CNAP1 antibody 14498-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. | ||
Nat Commun Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. | ||
Nat Commun Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis. | ||
PLoS Biol Stable centrosomal roots disentangle to allow interphase centriole independence. | ||
Proc Natl Acad Sci U S A Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins. | ||
J Cell Biol Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals. |
