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验证数据展示

  • IF Staining of HeLa using CL488-14498

    IF Staining of HeLa using CL488-14498

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 CEP250/CNAP1 antibody (CL488-14498) at dilution of 1:100, CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • IF Staining of MCF-7 using CL488-14498

    IF Staining of MCF-7 using CL488-14498

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite® Plus 488 CEP250/CNAP1 antibody (CL488-14498) at dilution of 1:100.

CoraLite® Plus 488-conjugated CEP250/CNAP1 Polyclonal antibody

CEP250/CNAP1 Polyclonal Antibody for IF/ICC
Cat No. CL488-14498

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CNAP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells, MCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 14498-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14498 targets CEP250/CNAP1 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen CEP250/CNAP1 fusion protein Ag5925 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centrosomal protein 250kDa
Synonyms 250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CNAP1
Calculated Molecular Weight 281 kDa
Observed Molecular Weight 250 kDa
GenBank Accession NumberBC001433
Gene Symbol CEP250/CNAP1
Gene ID (NCBI) 11190
RRIDAB_2919094
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV73
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP250, also known as C-Nap1, is a 250 kDa coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CEP250/CNAP1 antibody CL488-14498Download protocol
Standard Protocols
Click here to view our Standard Protocols