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验证数据展示

  • IF Staining of HeLa using CL594-14498

    IF Staining of HeLa using CL594-14498

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 CEP250/CNAP1 antibody (CL594-14498) at dilution of 1:100, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green).

  • IF Staining of MCF-7 using CL594-14498

    IF Staining of MCF-7 using CL594-14498

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite®594 CEP250/CNAP1 antibody (CL594-14498) at dilution of 1:100.

CoraLite®594-conjugated CEP250/CNAP1 Polyclonal antibody

CEP250/CNAP1 Polyclonal Antibody for IF/ICC
Cat No. CL594-14498

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CNAP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells, MCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 14498-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-14498 targets CEP250/CNAP1 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 CEP250/CNAP1 fusion protein Ag5925 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centrosomal protein 250kDa
别名 250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CNAP1
计算分子量 281 kDa
GenBank蛋白编号BC001433
基因名称 CEP250/CNAP1
Gene ID (NCBI) 11190
RRIDAB_2919808
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BV73
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP250, also known as C-Nap1, is a 250 kDa coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.

实验方案

Product Specific Protocols
IF protocol for CL594 CEP250/CNAP1 antibody CL594-14498Download protocol
Standard Protocols
Click here to view our Standard Protocols