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CEP63 Polyclonal antibody

CEP63 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 16268-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

WB, IF/ICC, IP, ELISA and More (1)

Centrosomal protein of 63 kDa, centrosomal protein 63kDa

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, mouse liver tissue, HeLa cells, mouse thymus tissue
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inMDCK cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16268-1-AP targets CEP63 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat, canine
文献引用反应性human, mouse
免疫原 CEP63 fusion protein Ag9335 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centrosomal protein 63kDa
别名 Centrosomal protein of 63 kDa, centrosomal protein 63kDa
计算分子量 703 aa, 81 kDa
观测分子量 57-75 kDa
GenBank蛋白编号BC014050
基因名称 CEP63
Gene ID (NCBI) 80254
RRIDAB_2077079
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96MT8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).

实验方案

Product Specific Protocols
WB protocol for CEP63 antibody 16268-1-APDownload protocol
IF protocol for CEP63 antibody 16268-1-APDownload protocol
IP protocol for CEP63 antibody 16268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Nat Cell Biol

Massive centriole production can occur in the absence of deuterosomes in multiciliated cells.

Authors - Olivier Mercey
mouseWB,IF

Neuron

Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.

Authors - Divya Jayaraman
humanWB, IF, IP

Dev Cell

A dual role for UVRAG in maintaining chromosomal stability independent of autophagy.

Authors - Zhen Zhao
  • KO Validated
mouseIF

Nat Commun

Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.

Authors - Munemasa Mori
humanIF

Nat Commun

Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.

Authors - Søs Grønbæk Holdgaard
humanIF

EMBO J

Multimodal mechanisms of human centriole engagement and disengagement

Authors - Kei K Ito
  • KD Validated
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