验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, mouse liver tissue, HeLa cells, mouse thymus tissue |
Positive IP detected in | HEK-293 cells |
Positive IF/ICC detected in | MDCK cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16268-1-AP targets CEP63 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IF, IP |
经测试反应性 | human, mouse, rat, canine |
文献引用反应性 | human, mouse |
免疫原 | CEP63 fusion protein Ag9335 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | centrosomal protein 63kDa |
别名 | Centrosomal protein of 63 kDa, centrosomal protein 63kDa |
计算分子量 | 703 aa, 81 kDa |
观测分子量 | 57-75 kDa |
GenBank蛋白编号 | BC014050 |
基因名称 | CEP63 |
Gene ID (NCBI) | 80254 |
RRID | AB_2077079 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q96MT8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).
实验方案
Product Specific Protocols | |
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WB protocol for CEP63 antibody 16268-1-AP | Download protocol |
IF protocol for CEP63 antibody 16268-1-AP | Download protocol |
IP protocol for CEP63 antibody 16268-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol Massive centriole production can occur in the absence of deuterosomes in multiciliated cells. | ||
Neuron Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate. | ||
Dev Cell A dual role for UVRAG in maintaining chromosomal stability independent of autophagy.
| ||
Nat Commun Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis. | ||
Nat Commun Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. | ||