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验证数据展示

  • IF Staining of MDCK using CL488-16268

    IF Staining of MDCK using CL488-16268

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite® Plus 488 CEP63 antibody (CL488-16268) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CEP63 Polyclonal antibody

CEP63 Polyclonal Antibody for IF/ICC
Cat No. CL488-16268

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

IF/ICC

Synonyms

centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMDCK cells
For other applications, we recommend the unconjugated version of this antibody, 16268-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16268 targets CEP63 in IF/ICC applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat, Canine
Immunogen CEP63 fusion protein Ag9335 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centrosomal protein 63kDa
Synonyms centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386
Calculated Molecular Weight 703 aa, 81 kDa
Observed Molecular Weight 57-75 kDa
GenBank Accession NumberBC014050
Gene Symbol CEP63
Gene ID (NCBI) 80254
RRIDAB_2923742
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MT8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CEP63 antibody CL488-16268Download protocol
Standard Protocols
Click here to view our Standard Protocols