验证数据展示
经过测试的应用
Positive IF/ICC detected in | MDCK cells |
For other applications, we recommend the unconjugated version of this antibody, 16268-1-AP
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-16268 targets CEP63 in IF/ICC applications and shows reactivity with human, mouse, rat, Canine samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse, rat, Canine |
免疫原 | CEP63 fusion protein Ag9335 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | centrosomal protein 63kDa |
别名 | centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386 |
计算分子量 | 703 aa, 81 kDa |
观测分子量 | 57-75 kDa |
GenBank蛋白编号 | BC014050 |
基因名称 | CEP63 |
Gene ID (NCBI) | 80254 |
RRID | AB_2923742 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q96MT8 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 CEP63 antibody CL488-16268 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |