验证数据展示
经过测试的应用
| Positive IF/ICC detected in | hTERT-RPE1 cells |
For other applications, we recommend the unconjugated version of this antibody, 16268-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-16268 targets CEP63 in IF/ICC applications and shows reactivity with human, mouse, rat, Canine samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat, Canine |
| 免疫原 | CEP63 fusion protein Ag9335 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | centrosomal protein 63kDa |
| 别名 | centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386 |
| 计算分子量 | 703 aa, 81 kDa |
| 观测分子量 | 57-75 kDa |
| GenBank蛋白编号 | BC014050 |
| 基因名称 | CEP63 |
| Gene ID (NCBI) | 80254 |
| RRID | AB_2919829 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96MT8 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 CEP63 antibody CL594-16268 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |