验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells, L02 cells |
Planning an IHC experiment? We recommend our IHCeasy CHCHD2 Ready-To-Use IHC Kit. CHCHD2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66302-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66302 targets CHCHD2 in IF/ICC applications and shows reactivity with human, pig, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, pig, rat |
| 免疫原 | CHCHD2 fusion protein Ag24219 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil-helix-coiled-coil-helix domain containing 2 |
| 别名 | C7orf17, CHCHD2, NS2TP |
| 计算分子量 | 151 aa, 16 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC003079 |
| 基因名称 | CHCHD2 |
| Gene ID (NCBI) | 51142 |
| RRID | AB_2883305 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9Y6H1 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHCHD2 is a widely expressed 17 kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. The function of the CHCH domain is not well understood, and the few characterized proteins that harbor this domain have diverse functions. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CHCHD2 antibody CL488-66302 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |