验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy CHCHD2 Ready-To-Use IHC Kit. CHCHD2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66302-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-66302 targets CHCHD2 in IF/ICC applications and shows reactivity with human, pig, rat samples.
| Tested Applications | IF/ICC Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, pig, rat |
| Cited Reactivity | human |
| Immunogen | CHCHD2 fusion protein Ag24219 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | coiled-coil-helix-coiled-coil-helix domain containing 2 |
| Synonyms | C7orf17, CHCHD2, NS2TP |
| Calculated Molecular Weight | 151 aa, 16 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC003079 |
| Gene Symbol | CHCHD2 |
| Gene ID (NCBI) | 51142 |
| RRID | AB_2883552 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9Y6H1 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHCHD2 is a widely expressed 17 kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. The function of the CHCH domain is not well understood, and the few characterized proteins that harbor this domain have diverse functions. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 CHCHD2 antibody CL594-66302 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |