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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CHCHD4 Polyclonal antibody

CHCHD4 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-21090

Print datasheet

Synonyms

CHCHD4, FLJ31709, MIA40

验证数据展示

  • IF Staining of HeLa using CL488-21090

    IF Staining of HeLa using CL488-21090

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 CHCHD4 antibody (CL488-21090) at dilution of 1:200.

  • IF Staining of A431 using CL488-21090

    IF Staining of A431 using CL488-21090

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 CHCHD4 antibody (CL488-21090) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells, A431 cells
For other applications, we recommend the unconjugated version of this antibody, 21090-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21090 targets CHCHD4 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen CHCHD4 fusion protein Ag15339 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil-helix-coiled-coil-helix domain containing 4
Synonyms CHCHD4, FLJ31709, MIA40
Calculated Molecular Weight 142 aa, 16 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC033775
Gene Symbol CHCHD4
Gene ID (NCBI) 131474
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N4Q1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CHCHD4 antibody CL488-21090Download protocol
Standard Protocols
Click here to view our Standard Protocols