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验证数据展示

  • IF Staining of HeLa using CL488-21090

    IF Staining of HeLa using CL488-21090

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 CHCHD4 antibody (CL488-21090) at dilution of 1:200.

  • IF Staining of A431 using CL488-21090

    IF Staining of A431 using CL488-21090

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 CHCHD4 antibody (CL488-21090) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CHCHD4 Polyclonal antibody

CHCHD4 Polyclonal Antibody for IF/ICC
Cat No. CL488-21090

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

CHCHD4, FLJ31709, MIA40

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells, A431 cells
For other applications, we recommend the unconjugated version of this antibody, 21090-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21090 targets CHCHD4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 CHCHD4 fusion protein Ag15339 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil-helix-coiled-coil-helix domain containing 4
别名 CHCHD4, FLJ31709, MIA40
计算分子量 142 aa, 16 kDa
观测分子量 22 kDa
GenBank蛋白编号BC033775
基因名称 CHCHD4
Gene ID (NCBI) 131474
RRIDAB_3672726
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N4Q1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CHCHD4 antibody CL488-21090Download protocol
Standard Protocols
Click here to view our Standard Protocols