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CIB1 Monoclonal antibody

CIB1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, bovine

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D2C12

Cat no : 67901-1-Ig

Print datasheet

Synonyms

Calmyrin, CIB, CIB1, CIBP, DNA PKcs interacting protein, Kinase interacting protein, KIP, KIP1, PRKDCIP, SIP2 28, SNK interacting protein 2 28

验证数据展示

  • WB analysis using 67901-1-Ig

    WB analysis using 67901-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67901-1-Ig (CIB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67901-1-Ig

    IHC staining of human breast cancer using 67901-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67901-1-Ig (CIB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CIB1 antibody included
  • IHC staining of human breast cancer using 67901-1-Ig

    IHC staining of human breast cancer using 67901-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67901-1-Ig (CIB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CIB1 antibody included

经过测试的应用

Positive WB detected inLNCaP cells, Hela cells, K-562 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, Human milk
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CIB1 Ready-To-Use IHC Kit. CIB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67901-1-Ig targets CIB1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, bovine samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat, bovine
Immunogen CIB1 fusion protein Ag31174 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name calcium and integrin binding 1 (calmyrin)
Synonyms Calmyrin, CIB, CIB1, CIBP, DNA PKcs interacting protein, Kinase interacting protein, KIP, KIP1, PRKDCIP, SIP2 28, SNK interacting protein 2 28
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC000846
Gene Symbol CIB1
Gene ID (NCBI) 10519
RRIDAB_2918657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99828
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CIB1 antibody 67901-1-IgDownload protocol
IHC protocol for CIB1 antibody 67901-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols