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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of Jurkat using CL488-67901

    FC experiment of Jurkat using CL488-67901

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CIB1 (CL488-67901, Clone:1D2C12) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated CIB1 Monoclonal antibody

CIB1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67901

产品说明书

CloneNo. 1D2C12

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat, bovine

应用

FC (Intra)

别名

Calmyrin, CIB, CIB1, CIBP, DNA PKcs interacting protein, Kinase interacting protein, KIP, KIP1, PRKDCIP, SIP2 28, SNK interacting protein 2 28

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CIB1 Ready-To-Use IHC Kit. CIB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67901-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67901 targets CIB1 in FC (Intra) applications and shows reactivity with Human, mouse, rat, bovine samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse, rat, bovine
免疫原 CIB1 fusion protein Ag31174 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 calcium and integrin binding 1 (calmyrin)
别名 Calmyrin, CIB, CIB1, CIBP, DNA PKcs interacting protein, Kinase interacting protein, KIP, KIP1, PRKDCIP, SIP2 28, SNK interacting protein 2 28
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC000846
基因名称 CIB1
Gene ID (NCBI) 10519
RRIDAB_2923821
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99828
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CIB1 antibody CL488-67901Download protocol
Standard Protocols
Click here to view our Standard Protocols