CIN85 Monoclonal antibody

CIN85 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C12D2

Cat no : 66571-1-Ig

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Synonyms

CD2 binding protein 3, CD2BP3, CIN85, GIG10, HSB 1, MIG18, SH3KBP1



经过测试的应用

Positive WB detected inHL-60 cells, rat brain tissue, K-562 cells, PC-12 cells, mouse brain tissue, THP-1 cells, Jurkat cells, Ramos cells, Raji cells, Daudi cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66571-1-Ig targets CIN85 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen CIN85 fusion protein Ag2777 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SH3-domain kinase binding protein 1
Synonyms CD2 binding protein 3, CD2BP3, CIN85, GIG10, HSB 1, MIG18, SH3KBP1
Calculated Molecular Weight 665 aa, 73 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC015806
Gene Symbol SH3KBP1/CIN85
Gene ID (NCBI) 30011
RRIDAB_2881932
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96B97
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.

实验方案

Product Specific Protocols
WB protocol for CIN85 antibody 66571-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols