验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, THP-1 cells, HL-60 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66571 targets CIN85 in WB applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | CIN85 fusion protein Ag2777 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SH3-domain kinase binding protein 1 |
| 别名 | CD2 binding protein 3, CD2BP3, CIN85, GIG10, HSB 1, MIG18, SH3KBP1 |
| 计算分子量 | 665 aa, 73 kDa |
| 观测分子量 | 85 kDa |
| GenBank蛋白编号 | BC015806 |
| 基因名称 | SH3KBP1/CIN85 |
| Gene ID (NCBI) | 30011 |
| RRID | AB_3084260 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96B97 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 CIN85 antibody CL488-66571 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |