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CLN3 Monoclonal antibody

CLN3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E10A9

Cat no : 67957-1-Ig

Print datasheet

Synonyms

Batten disease protein, BATTENIN, BTS, CLN3, JNCL, Protein CLN3

验证数据展示

  • WB analysis using 67957-1-Ig

    WB analysis using 67957-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67957-1-Ig (CLN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, NCCIT cells, NCI-H1299 cells, A549 cells, Jurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67957-1-Ig targets CLN3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen CLN3 fusion protein Ag31402 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ceroid-lipofuscinosis, neuronal 3
Synonyms Batten disease protein, BATTENIN, BTS, CLN3, JNCL, Protein CLN3
Calculated Molecular Weight 438 aa, 48 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC002394
Gene Symbol CLN3
Gene ID (NCBI) 1201
RRIDAB_2918708
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13286
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuronal ceroid lipofuscinosis (NCL, or Batten disease) refers to a group of lethal pediatric neurodegenerative diseases originating from mutations in one of the thus far identified 13 CLN genes (Ceroid Lipofuscinosis, Neuronal type; CLN1 to CLN14) (PMID: 25051496). CLN3 is a multi-membrane-spanning protein involved in the microtubule-dependent, anterograde transport of late endosomes and lysosomes. The CLN3 gene is located on chromosome 16p12.1 and produces three mRNA splicing variants. The 438-amino-acid CLN3 protein has a calculated molecular weight of 48 kDa. It has been reported that CLN3 can be glycosylated and form a homodimeric complex (PMID: 10356317; 17286803).

实验方案

Product Specific Protocols
WB protocol for CLN3 antibody 67957-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols