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验证数据展示

  • FC experiment of HeLa using CL488-67957

    FC experiment of HeLa using CL488-67957

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CLN3 (CL488-67957, Clone:1E10A9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated CLN3 Monoclonal antibody

CLN3 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67957

产品说明书

CloneNo. 1E10A9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

Protein CLN3, JNCL, BTS, BATTENIN, Batten disease protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67957-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67957 targets CLN3 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 CLN3 fusion protein Ag31402 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ceroid-lipofuscinosis, neuronal 3
别名 Protein CLN3, JNCL, BTS, BATTENIN, Batten disease protein
计算分子量 438 aa, 48 kDa
观测分子量50 kDa
GenBank蛋白编号BC002394
基因名称 CLN3
Gene ID (NCBI) 1201
RRIDAB_2934599
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13286
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuronal ceroid lipofuscinosis (NCL, or Batten disease) refers to a group of lethal pediatric neurodegenerative diseases originating from mutations in one of the thus far identified 13 CLN genes (Ceroid Lipofuscinosis, Neuronal type; CLN1 to CLN14) (PMID: 25051496). CLN3 is a multi-membrane spanning protein that is involved in microtubule-dependent, anterograde transport of late endosomes and lysosomes. The CLN3 gene is located on chromosome 16p12.1and produces three mRNA splicing variants. The 438-amino-acid CLN3 protein has a calculated molecular weight of 48 kDa. It has been reported that CLN3 can be glycosylated and form homodimeric complex (PMID: 10356317; 17286803).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CLN3 antibody CL488-67957Download protocol
Standard Protocols
Click here to view our Standard Protocols