CoraLite® Plus 488-conjugated CLN3 Monoclonal antibody
CLN3 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E10A9
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 67957-1-Ig
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67957 targets CLN3 in FC (Intra) applications and shows reactivity with human samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | CLN3 fusion protein Ag31402 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | ceroid-lipofuscinosis, neuronal 3 |
Synonyms | Protein CLN3, JNCL, BTS, BATTENIN, Batten disease protein |
Calculated Molecular Weight | 438 aa, 48 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC002394 |
Gene Symbol | CLN3 |
Gene ID (NCBI) | 1201 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q13286 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neuronal ceroid lipofuscinosis (NCL, or Batten disease) refers to a group of lethal pediatric neurodegenerative diseases originating from mutations in one of the thus far identified 13 CLN genes (Ceroid Lipofuscinosis, Neuronal type; CLN1 to CLN14) (PMID: 25051496). CLN3 is a multi-membrane spanning protein that is involved in microtubule-dependent, anterograde transport of late endosomes and lysosomes. The CLN3 gene is located on chromosome 16p12.1and produces three mRNA splicing variants. The 438-amino-acid CLN3 protein has a calculated molecular weight of 48 kDa. It has been reported that CLN3 can be glycosylated and form homodimeric complex (PMID: 10356317; 17286803).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 CLN3 antibody CL488-67957 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |