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验证数据展示

  • IF Staining of HeLa using CL488-10455

    IF Staining of HeLa using CL488-10455

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 CLTB antibody (CL488-10455) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CLTB Polyclonal antibody

CLTB Polyclonal Antibody for IF/ICC
Cat No. CL488-10455

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CLTB Ready-To-Use IHC Kit. CLTB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10455-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10455 targets CLTB in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen CLTB fusion protein Ag0707 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name clathrin, light chain (Lcb)
Synonyms Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 32-38 kDa
GenBank Accession NumberBC006457
Gene Symbol CLTB
Gene ID (NCBI) 1212
RRIDAB_3672443
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09497
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CLTB antibody CL488-10455Download protocol
Standard Protocols
Click here to view our Standard Protocols