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验证数据展示

  • IF Staining of HeLa using CL488-10455

    IF Staining of HeLa using CL488-10455

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 CLTB antibody (CL488-10455) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CLTB Polyclonal antibody

CLTB Polyclonal Antibody for IF/ICC
Cat No. CL488-10455

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CLTB Ready-To-Use IHC Kit. CLTB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10455-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10455 targets CLTB in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 CLTB fusion protein Ag0707 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 clathrin, light chain (Lcb)
别名 Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB
计算分子量 23 kDa
观测分子量 32-38 kDa
GenBank蛋白编号BC006457
基因名称 CLTB
Gene ID (NCBI) 1212
RRIDAB_3672443
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09497
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CLTB antibody CL488-10455Download protocol
Standard Protocols
Click here to view our Standard Protocols