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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CLTC Polyclonal antibody

CLTC Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26523

Print datasheet

Synonyms

CLH 17, clathrin, heavy chain (Hc), Clathrin heavy chain 1, Clathrin heavy chain, CHC17

验证数据展示

  • IF Staining of HeLa using CL488-26523

    IF Staining of HeLa using CL488-26523

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 CLTC antibody (CL488-26523) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-26523

    FC experiment of HepG2 using CL488-26523

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 CLTC Polyclonal Antibody (CL488-26523)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CLTC Ready-To-Use IHC Kit. CLTC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26523-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26523 targets CLTC in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen CLTC fusion protein Ag25041 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name clathrin, heavy chain (Hc)
Synonyms CLH 17, clathrin, heavy chain (Hc), Clathrin heavy chain 1, Clathrin heavy chain, CHC17
Calculated Molecular Weight 192 kDa
Observed Molecular Weight 180 kDa
GenBank Accession NumberBC054489
Gene Symbol CLTC
Gene ID (NCBI) 1213
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00610
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CLTC antibody CL488-26523Download protocol
Standard Protocols
Click here to view our Standard Protocols