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CoraLite® Plus 488-conjugated CNOT4 Monoclonal antibody

CNOT4 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4H5H3

Cat no : CL488-67798

Print datasheet

Synonyms

E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4

验证数据展示

  • IF Staining of human colon cancer using CL488-67798

    IF Staining of human colon cancer using CL488-67798

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 CNOT4 antibody (CL488-67798, Clone: 4H5H3 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL488-67798

    IF Staining of HepG2 using CL488-67798

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CNOT4 antibody (CL488-67798, Clone: 4H5H3 ) at dilution of 1:800.

经过测试的应用

Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CNOT4 Ready-To-Use IHC Kit. CNOT4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67798-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67798 targets CNOT4 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen CNOT4 fusion protein Ag29964 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CCR4-NOT transcription complex, subunit 4
Synonyms E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4
Calculated Molecular Weight 575 aa, 64 kDa
GenBank Accession NumberBC035590
Gene Symbol CNOT4
Gene ID (NCBI) 4850
RRIDAB_2919550
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95628
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CNOT4 antibody CL488-67798Download protocol
Standard Protocols
Click here to view our Standard Protocols