验证数据展示
经过测试的应用
Positive IF-P detected in | human colon cancer tissue |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67798 targets CNOT4 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF/ICC, IF-P Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | CNOT4 fusion protein Ag29964 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | CCR4-NOT transcription complex, subunit 4 |
别名 | E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4 |
计算分子量 | 575 aa, 64 kDa |
GenBank蛋白编号 | BC035590 |
基因名称 | CNOT4 |
Gene ID (NCBI) | 4850 |
RRID | AB_2919550 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | O95628 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 CNOT4 antibody CL488-67798 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |