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CoraLite®594-conjugated CNOT4 Monoclonal antibody

CNOT4 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4H5H3

Cat no : CL594-67798

Print datasheet

Synonyms

CCR4 associated factor 4, CLONE243, CNOT4, NOT4, NOT4H

验证数据展示

  • IF Staining of human colon cancer using CL594-67798

    IF Staining of human colon cancer using CL594-67798

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 CNOT4 antibody (CL594-67798, Clone: 4H5H3 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CNOT4 Ready-To-Use IHC Kit. CNOT4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67798-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67798 targets CNOT4 in IF-P applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, mouse, rat
Immunogen CNOT4 fusion protein Ag29964 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CCR4-NOT transcription complex, subunit 4
Synonyms CCR4 associated factor 4, CLONE243, CNOT4, NOT4, NOT4H
Calculated Molecular Weight 575 aa, 64 kDa
GenBank Accession NumberBC035590
Gene Symbol CNOT4
Gene ID (NCBI) 4850
RRIDAB_2920185
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95628
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.

实验方案

Product Specific Protocols
IF protocol for CL594 CNOT4 antibody CL594-67798Download protocol
Standard Protocols
Click here to view our Standard Protocols