验证数据展示
经过测试的应用
| Positive IF-P detected in | human colon cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy CNOT4 Ready-To-Use IHC Kit. CNOT4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67798-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67798 targets CNOT4 in IF-P applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | CNOT4 fusion protein Ag29964 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CCR4-NOT transcription complex, subunit 4 |
| 别名 | CCR4 associated factor 4, CLONE243, CNOT4, NOT4, NOT4H |
| 计算分子量 | 575 aa, 64 kDa |
| GenBank蛋白编号 | BC035590 |
| 基因名称 | CNOT4 |
| Gene ID (NCBI) | 4850 |
| RRID | AB_2920185 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O95628 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 CNOT4 antibody CL594-67798 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |