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验证数据展示

  • IF Staining of HepG2 using CL488-83615-4

    IF Staining of HepG2 using CL488-83615-4

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CNOT4 antibody (CL488-83615-4, Clone: 240559E1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CNOT4 Recombinant antibody

CNOT4 Recombinant Antibody for IF/ICC
Cat No. CL488-83615-4

产品说明书

CloneNo. 240559E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CNOT4 Ready-To-Use IHC Kit. CNOT4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83615-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83615-4 targets CNOT4 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 CNOT4 fusion protein Ag3163 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CCR4-NOT transcription complex, subunit 4
别名 E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4
计算分子量 575 aa, 64 kDa
观测分子量 64-85 kDa
GenBank蛋白编号BC035590
基因名称 CNOT4
Gene ID (NCBI) 4850
RRIDAB_3673282
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95628
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CNOT4 antibody CL488-83615-4Download protocol
Standard Protocols
Click here to view our Standard Protocols