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验证数据展示

  • IF Staining of HepG2 using CL488-83615-4

    IF Staining of HepG2 using CL488-83615-4

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CNOT4 antibody (CL488-83615-4, Clone: 240559E1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CNOT4 Recombinant antibody

CNOT4 Recombinant Antibody for IF/ICC
Cat No. CL488-83615-4

Print datasheet

CloneNo. 240559E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CNOT4 Ready-To-Use IHC Kit. CNOT4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83615-4-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83615-4 targets CNOT4 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen CNOT4 fusion protein Ag3163 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CCR4-NOT transcription complex, subunit 4
Synonyms E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4
Calculated Molecular Weight 575 aa, 64 kDa
Observed Molecular Weight 64-85 kDa
GenBank Accession NumberBC035590
Gene Symbol CNOT4
Gene ID (NCBI) 4850
RRIDAB_3673282
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95628
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CNOT4 antibody CL488-83615-4Download protocol
Standard Protocols
Click here to view our Standard Protocols