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验证数据展示

  • WB analysis of human skeletal muscle using 66824-1-Ig

    WB analysis of human skeletal muscle using 66824-1-Ig

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66824-1-Ig

    IHC staining of human liver cancer using 66824-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL6A1 antibody included
  • IHC staining of human liver cancer using 66824-1-Ig

    IHC staining of human liver cancer using 66824-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL6A1 antibody included
  • IF Staining of human liver cancer using 66824-1-Ig

    IF Staining of human liver cancer using 66824-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 66824-1-Ig

    IF Staining of human liver cancer using 66824-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 66824-1-Ig

    IF Staining of human liver cancer using 66824-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

COL6A1 Monoclonal antibody

COL6A1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66824-1-Ig

产品说明书

CloneNo. 3H10B1

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

COL6, 3H10B1, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, human heart tissue
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy COL6A1 Ready-To-Use IHC Kit. COL6A1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66824-1-Ig targets COL6A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 COL6A1 fusion protein Ag11809 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 collagen, type VI, alpha 1
别名 COL6, 3H10B1, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain
计算分子量 1028 aa, 109 kDa
观测分子量 140-150 kDa
GenBank蛋白编号BC052575
基因名称 COL6A1
Gene ID (NCBI) 1291
RRIDAB_2882167
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12109
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

实验方案

Product Specific Protocols
WB protocol for COL6A1 antibody 66824-1-IgDownload protocol
IHC protocol for COL6A1 antibody 66824-1-IgDownload protocol
IF protocol for COL6A1 antibody 66824-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols