CoraLite® Plus 488-conjugated Collagen Type VI Monoclonal antibody
Collagen Type VI Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3H10B1
验证数据展示
经过测试的应用
| Positive IF-P detected in | human liver cancer tissue, human colon cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy COL6A1 Ready-To-Use IHC Kit. COL6A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66824-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66824 targets Collagen Type VI in IF-P applications and shows reactivity with Human samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | Human |
| Immunogen | Collagen Type VI fusion protein Ag11809 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | collagen, type VI, alpha 1 |
| Synonyms | COL6A1, Collagen alpha 1(VI) chain, Collagen Type VI , collagen, type VI, alpha 1, OPLL |
| Calculated Molecular Weight | 1028 aa, 109 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC052575 |
| Gene Symbol | COL6A1 |
| Gene ID (NCBI) | 1291 |
| RRID | AB_2919376 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P12109 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Collagen Type VI antibody CL488-66824 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |