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CoraLite®594-conjugated Collagen Type VI Monoclonal antibody

Collagen Type VI Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3H10B1

Cat no : CL594-66824

Print datasheet

Synonyms

COL6A1, Collagen alpha 1(VI) chain, Collagen Type VI , collagen, type VI, alpha 1, OPLL

验证数据展示

  • IF Staining of human liver cancer using CL594-66824

    IF Staining of human liver cancer using CL594-66824

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Collagen Type VI antibody (CL594-66824, Clone: 3H10B1 ) at dilution of 1:200.

  • IF Staining of human liver cancer using CL594-66824

    IF Staining of human liver cancer using CL594-66824

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Collagen Type VI antibody (CL594-66824, Clone: 3H10B1 ) at dilution of 1:200.

  • IF Staining of human colon cancer using CL594-66824

    IF Staining of human colon cancer using CL594-66824

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Collagen Type VI antibody (CL594-66824, Clone: 3H10B1 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman liver cancer tissue, human colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy COL6A1 Ready-To-Use IHC Kit. COL6A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66824-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66824 targets Collagen Type VI in IF-P applications and shows reactivity with Human samples.

Tested Applications IF-P Application Description
Tested Reactivity Human
Immunogen Collagen Type VI fusion protein Ag11809 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name collagen, type VI, alpha 1
Synonyms COL6A1, Collagen alpha 1(VI) chain, Collagen Type VI , collagen, type VI, alpha 1, OPLL
Calculated Molecular Weight 1028 aa, 109 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC052575
Gene Symbol COL6A1
Gene ID (NCBI) 1291
RRIDAB_2920045
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12109
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

实验方案

Product Specific Protocols
IF protocol for CL594 Collagen Type VI antibody CL594-66824Download protocol
Standard Protocols
Click here to view our Standard Protocols