CoraLite®594-conjugated COPS2 Monoclonal antibody
COPS2 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2H10E6
验证数据展示
经过测试的应用
| Positive IF-P detected in | human ovary cancer cells |
Planning an IHC experiment? We recommend our IHCeasy COPS2 Ready-To-Use IHC Kit. COPS2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66435-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66435 targets COPS2 in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | COPS2 fusion protein Ag1415 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis) |
| Synonyms | ALIEN, Alien homolog, COPS2, CSN2, SGN2, Signalosome subunit 2, TR interacting protein 15, TRIP 15, TRIP15 |
| Calculated Molecular Weight | 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC012629 |
| Gene Symbol | COPS2 |
| Gene ID (NCBI) | 9318 |
| RRID | AB_2919990 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P61201 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 COPS2 antibody CL594-66435 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |