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CoraLite® Plus 488-conjugated CPLX1 Polyclonal antibody

CPLX1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10246

Print datasheet

Synonyms

Synaphin 2, CPXI, CPX1, CPX I, CPLX 1

验证数据展示

  • IF Staining of SH-SY5Y using CL488-10246

    IF Staining of SH-SY5Y using CL488-10246

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 CPLX1 antibody (CL488-10246) at dilution of 1:200.

  • FC experiment of SH-SY5Y using CL488-10246

    FC experiment of SH-SY5Y using CL488-10246

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Cplx1 Polyclonal Antibody (CL488-10246)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10246 targets CPLX1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CPLX1 fusion protein Ag0387 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complexin 1
Synonyms Synaphin 2, CPXI, CPX1, CPX I, CPLX 1
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 15-20 kDa
GenBank Accession NumberBC002471
Gene Symbol CPLX1
Gene ID (NCBI) 10815
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14810
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Complexin 1 (CPLX1) is a member of the complexin/synaphin gene family, which are cytosolic proteins that function in synaptic vesicle exocytosis. Complexins are enriched in neurons where they colocalize with syntaxin and SNAP-25. Complexins bind weakly to syntaxin alone and not at all to synaptobrevin and SNAP-25, but strongly to the SNAP receptor-core complex composed of these three molecules. Compexins also compete with alpha-SNAP for binding to the core complex but not with other interacting molecules, including synaptotagmin I, and regulate the sequential interactions of alpha-SNAP and synaptotagmins with the SNAP receptor during exocytosis. CLPX1 binds to the SNAP receptor complex and disrupts it, leading to the release of transmitters. Alterations of complexins may contribute to the molecular substrate for abnormalities of neural connectivity in severe mental disorders.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CPLX1 antibody CL488-10246Download protocol
FC protocol for CL Plus 488 CPLX1 antibody CL488-10246Download protocol
Standard Protocols
Click here to view our Standard Protocols