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验证数据展示

  • FC experiment of HeLa using CL647-66039

    FC experiment of HeLa using CL647-66039

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human CPT1A (CL647-66039, Clone:5D8G9) (red), or 0.4 ug CoraLite® Plus 647 Mouse Mouse IgG2a isotype control (11A1B2) (CL647-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated CPT1A Monoclonal antibody

CPT1A Monoclonal Antibody for FC (Intra)
Cat No. CL647-66039

产品说明书

CloneNo. 5D8G9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CPT1A Ready-To-Use IHC Kit. CPT1A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66039-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66039 targets CPT1A in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 CPT1A fusion protein Ag7745 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 carnitine palmitoyltransferase 1A (liver)
别名 CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1
计算分子量 88 kDa
观测分子量 86 kDa
GenBank蛋白编号BC000185
基因名称 CPT1A
Gene ID (NCBI) 1374
RRIDAB_2920275
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50416
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CPT1A antibody CL647-66039Download protocol
Standard Protocols
Click here to view our Standard Protocols