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  • Featured Product
  • KD/KO Validated

CRACR2A Monoclonal antibody, PBS Only

CRACR2A Monoclonal Antibody for WB,Indirect ELISA,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

4C1F5

Cat No : 66787-1-PBS

Print datasheet

Synonyms

CRAC channel regulator 2A, CRACR2A, EFCAB4B, Rab46

验证数据展示

  • WB analysis using 66787-1-Ig (same clone as 66787-1-PBS)

    WB analysis using 66787-1-Ig (same clone as 66787-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66787-1-Ig (CRACR2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66787-1-Ig (same clone as 66787-1-PBS)

    WB analysis of Jurkat using 66787-1-Ig (same clone as 66787-1-PBS)

    WB result of CRACR2A antibody (66787-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRACR2A transfected Jurkat cells. This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66787-1-Ig (same clone as 66787-1-PBS)

    IF Staining of HEK-293 using 66787-1-Ig (same clone as 66787-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66787-1-Ig (CRACR2A antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

产品信息

66787-1-PBS targets CRACR2A in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human
Immunogen CRACR2A fusion protein Ag7525 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name EF-hand calcium binding domain 4B
Synonyms CRAC channel regulator 2A, CRACR2A, EFCAB4B, Rab46
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC004524
Gene Symbol EFCAB4B
Gene ID (NCBI) 84766
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BSW2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.