• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66787-1-Ig (same clone as 66787-1-PBS)

    WB analysis using 66787-1-Ig (same clone as 66787-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66787-1-Ig (CRACR2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66787-1-Ig (same clone as 66787-1-PBS)

    WB analysis of Jurkat using 66787-1-Ig (same clone as 66787-1-PBS)

    WB result of CRACR2A antibody (66787-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRACR2A transfected Jurkat cells. This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66787-1-Ig (same clone as 66787-1-PBS)

    IF Staining of HEK-293 using 66787-1-Ig (same clone as 66787-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66787-1-Ig (CRACR2A antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66787-1-Ig (same clone as 66787-1-PBS)

    IHC staining of human colon using 66787-1-Ig (same clone as 66787-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66787-1-Ig (CRACR2A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CRACR2A Monoclonal antibody, PBS Only

CRACR2A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66787-1-PBS

产品说明书

CloneNo. 4C1F5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EFCAB4B, EF-hand calcium-binding domain-containing protein 4B, CRAC channel regulator 2A, Calcium release-activated channel regulator 2A, Calcium release-activated calcium channel regulator 2A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

66787-1-PBS targets CRACR2A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 CRACR2A fusion protein Ag7525 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 EF-hand calcium binding domain 4B
别名 EFCAB4B, EF-hand calcium-binding domain-containing protein 4B, CRAC channel regulator 2A, Calcium release-activated channel regulator 2A, Calcium release-activated calcium channel regulator 2A
计算分子量 46 kDa
观测分子量 80-90 kDa
GenBank蛋白编号BC004524
基因名称 CRACR2A
Gene ID (NCBI) 84766
RRIDAB_2882132
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BSW2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.